Enhanced Cellulase Production by Talaromyces amestolkiae CMIAT055 Using Banana Pseudostem

Cellulases are a complex of enzymes necessary for the complete solubilization cellulose in sugars, thus playing key role natural carbon cycle through hydrolysis lignocellulosic structures. The aim this study was to evaluate increase capacity Talaromyces amestolkiae CMIAT 055 produce cellulases by optimizing components culture medium containing banana pseudostem as an inducer, well different agitation configurations bioreactor. Optimization performed statistical experimental design (Plackett–Burman and DCCR), pH control bioreactors, system comparing impellers with flow profiles liquid medium. For purpose, wild strain used. In Plackett–Burman DCCR design, four were significant optimized greater synthesis FPase: pseudostem, CaCl2, KH2PO4, urea. bioreactors tests, these parameters beneficial enzyme activities: maintenance at 5.0, use Pitched blade impeller, rotation speed 300 rpm. Comparing first test using Erlenmeyer flask last fermentation process it observed that total cellulase activity increased from 424.7 2172.8 FPU/L. This fact showed strategies adopted pertinent way reduce cost production materials.Graphical Abstract